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anti ago2 antibodies  (Boster Bio)
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Boster Bio anti ago2 antibodies
A , B Distribution of NDRG1-OT1 in MDA-MB-231 cells under normoxia ( A ) and hypoxia ( B ). Cytoplasmic and nuclear RNA were fractionated in MDA-MB-231 cells under normoxia ( A ) and hypoxia ( B ). Relative abundance of RNA was normalized to the total amount of RNA and detected by qPCR. GAPDH : cytoplasmic marker; BCAR4 : nuclear marker. C Relative expression levels of six predicted miRNAs in MDA-MB-231 cells overexpressing NDRG1-OT1 . The candidate miRNAs binding NDRG1-OT1 were predicted by miRDB ( http://mirdb.org/ ). The expression levels of miRNAs were measured by qPCR and normalized to U6 . D RIP analysis of NDRG1-OT1 using antibody against <t>AGO2</t> in normoxia. The RIP enrichment of the AGO2-associated lncRNA was measured by qPCR and normalized to 18 S rRNA. The relative fold enrichment was calculated as compared to the IgG group. E Expression levels of miR-875-3p in five breast cancer cell lines under hypoxia and normoxia, detected by qPCR. Loading control: U6 snRNA. The relative expression levels in each cell line were compared with those of the normoxic group, respectively. F Relative expression of NDRG1-OT1 in MDA-MB-231 cells overexpressing miR-875-3p , and followed by treatment of miR-875-3p inhibitor (50 nM). Loading control: GAPDH . ( n = 5). G Schematic representation of firefly luciferase constructs containing the sequence of NDRG1-OT1 (WT) and two mutations of miR-875-3p binding sites (sites 1 and 2). H Luciferase reporter assays of NDRG1-OT1 in HEK293T cells overexpressing miR-875-3p . All data shown are the means ± SDs. * P < 0.05.
Anti Ago2 Antibodies, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ago2+rabbit+monoclonal+antibody/Anti-Argonaute+2+AGO2+Rabbit+Monoclonal+Antibody/pmc09489765-123-34-39
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anti ago2 antibodies - by Bioz Stars, 2026-10
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A , B Distribution of NDRG1-OT1 in MDA-MB-231 cells under normoxia ( A ) and hypoxia ( B ). Cytoplasmic and nuclear RNA were fractionated in MDA-MB-231 cells under normoxia ( A ) and hypoxia ( B ). Relative abundance of RNA was normalized to the total amount of RNA and detected by qPCR. GAPDH : cytoplasmic marker; BCAR4 : nuclear marker. C Relative expression levels of six predicted miRNAs in MDA-MB-231 cells overexpressing NDRG1-OT1 . The candidate miRNAs binding NDRG1-OT1 were predicted by miRDB ( http://mirdb.org/ ). The expression levels of miRNAs were measured by qPCR and normalized to U6 . D RIP analysis of NDRG1-OT1 using antibody against AGO2 in normoxia. The RIP enrichment of the AGO2-associated lncRNA was measured by qPCR and normalized to 18 S rRNA. The relative fold enrichment was calculated as compared to the IgG group. E Expression levels of miR-875-3p in five breast cancer cell lines under hypoxia and normoxia, detected by qPCR. Loading control: U6 snRNA. The relative expression levels in each cell line were compared with those of the normoxic group, respectively. F Relative expression of NDRG1-OT1 in MDA-MB-231 cells overexpressing miR-875-3p , and followed by treatment of miR-875-3p inhibitor (50 nM). Loading control: GAPDH . ( n = 5). G Schematic representation of firefly luciferase constructs containing the sequence of NDRG1-OT1 (WT) and two mutations of miR-875-3p binding sites (sites 1 and 2). H Luciferase reporter assays of NDRG1-OT1 in HEK293T cells overexpressing miR-875-3p . All data shown are the means ± SDs. * P < 0.05.

Journal: Cell Death & Disease

Article Title: Regulatory mechanisms and function of hypoxia-induced long noncoding RNA NDRG1-OT1 in breast cancer cells

doi: 10.1038/s41419-022-05253-2

Figure Lengend Snippet: A , B Distribution of NDRG1-OT1 in MDA-MB-231 cells under normoxia ( A ) and hypoxia ( B ). Cytoplasmic and nuclear RNA were fractionated in MDA-MB-231 cells under normoxia ( A ) and hypoxia ( B ). Relative abundance of RNA was normalized to the total amount of RNA and detected by qPCR. GAPDH : cytoplasmic marker; BCAR4 : nuclear marker. C Relative expression levels of six predicted miRNAs in MDA-MB-231 cells overexpressing NDRG1-OT1 . The candidate miRNAs binding NDRG1-OT1 were predicted by miRDB ( http://mirdb.org/ ). The expression levels of miRNAs were measured by qPCR and normalized to U6 . D RIP analysis of NDRG1-OT1 using antibody against AGO2 in normoxia. The RIP enrichment of the AGO2-associated lncRNA was measured by qPCR and normalized to 18 S rRNA. The relative fold enrichment was calculated as compared to the IgG group. E Expression levels of miR-875-3p in five breast cancer cell lines under hypoxia and normoxia, detected by qPCR. Loading control: U6 snRNA. The relative expression levels in each cell line were compared with those of the normoxic group, respectively. F Relative expression of NDRG1-OT1 in MDA-MB-231 cells overexpressing miR-875-3p , and followed by treatment of miR-875-3p inhibitor (50 nM). Loading control: GAPDH . ( n = 5). G Schematic representation of firefly luciferase constructs containing the sequence of NDRG1-OT1 (WT) and two mutations of miR-875-3p binding sites (sites 1 and 2). H Luciferase reporter assays of NDRG1-OT1 in HEK293T cells overexpressing miR-875-3p . All data shown are the means ± SDs. * P < 0.05.

Article Snippet: The lysate was centrifuged at 14,000 rpm for 10 min. Then, 900 μL RIP immunoprecipitation buffer (Millipore) containing RIP wash buffer, 0.5% EDTA, and RNase inhibitor were added to the supernatant with 5 μg anti-AGO2 antibodies (cat. no. M00189; Boster Biological Technology, Pleasanton, CA, USA) or 5 μg anti-IgG control antibodies (cat. no. AQ127; Millipore) that were prebound on magnetic beads.

Techniques: Marker, Expressing, Binding Assay, Control, Luciferase, Construct, Sequencing